Microscope X versus Microscope Y versus Microscope Z


Comparison of three different muscle spindles from YPF expressing mice (Thy1 promoter) using as identical settings & conditions as possible on three different confocal microscopes.  Each image is the maximum projection of a z-series sample.  Step size 0.44 um.  Note that the same algorithm was used for each maximum projection.  Note that substantial differences in the image produced by each manufacturers' projection was noted.  See these images for comparison.  


Microscope X Muscle Spindle maximum projection 40X oil NA 1.3  z=1 2048 scan length=pixels pinhole=1 Airy Disk
 

Microscope Y Muscle Spindle maximum projection 40X oil NA 1.3  z=1 2048 scan length=pixels pinhole=1 Airy Disk
 

Microscope Z Muscle Spindle maximum projection 40X oil NA 1.3  z=1 2048 scan length=pixels pinhole=1 Airy Disk
 

 

Microscope X Microscope Y
10xgdnf2_mp-x.gif (23806 bytes)  10X  
25X oil

20X oil

40xgdnf_mp-x.gif (15357 bytes) 40x   Z-Series movie -> Movie Windows AVI Movie Mac QuickTime