8.2.1  Convert individual Leica tiff files into bitmap files using tiffsplit4.exe 

• Open a DOS box  -  (8.2.1.1 Some useful DOS box commands)
• Change to the drive with the tiff image file e.g. type  e:  enter
• Change to the directory which contain the tiff image file e.g. type  cd \pictures\sim  enter
• Convert tiff file into a bitmaps series e.g. type  tiffsplit4  sim.tif  enter
• Or:  tiffsplit4 sim.tif -e  enter  (if exntended focus images required as well)
• A series of bitmap files will be created for each channel from the tiff file(s) and are placed in the same directory

8.2.2  Convert the bitmap series for each channel into Biorad PIC format file(s) using bmp2pic.exe

• Use the already open DOS box (or re-open one and change to drive and directory)
• Execute:  bmp2pic filename_a*.bmp  to convert the 1st channel bitmaps into a pic file
• Convert other channels, if any using:  bmp2pic filename_b*.bmp  to produce the pic file for the second channel.

8.2.3  The bitmap file series created may be deleted now (if they are not going to be used for T3D, or Slicer, or creating an extended focus with bmpsum.exe, or etc.).  

• Execute:  del filename_*.bmp or click selected and deleted in "My Computer" or "File Manager".  
• Note: be careful if you have generated extended focus image(s) with tiffsplit4.exe which will have made filename_xf_a.bmp etc., which will be erased by: del filename_*.bmp.
  • Instead do:
    del filename_a*.bmp,
    del filename_b*.bmp  
    etc. for each channel instead, to preserve filename_xf_?.bmp

Go to 8.1   Using Confocal Assistant an overview

Go to 8.3  Load PIC file into Confocal Assistant, Viewing the z-series as a movie, generating a maximum brightness projection.

Last updated 10-24-2006

					© 1998-2003 Michael Chua, Cell & Molecular Physiology, UNC.  All rights reserved.
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