Overview of the Physiology Imaging and Neuroscience Confocal Facility


1.  Available Equipment

1.1  A LeicaTCS-NT confocal laser scanning confocal microscope with:

1.2  A general purpose PC (\\ORAC) is available for image processing, analysis, figure preparation, movie production, CD-R & CD-RW recording.  This equipment is provided by the Department of Cell & Molecular Physiology’s high speed photo diode imaging array.  Confocal users from all departments have been invited to utilize this resource.  This computer is also used for program development and the preparation of confocal usage reports and bills.  Software on this system includes: Adobe  Photoshop 5.0, ThumbsPlus 4, Tiffsplit4, VidEdit, Slicer, Voxblast, ImagePro+, EasyCD Creator 4, WinZip, LViewPro 2.1, Premiere 4.2, Cas 4.02 and various programs for handling Bitmap Image series.

1.3  A PC for Windows 2000 Server domain control services (PHYSIOLOGY domain), which is used to store confocal user account information and logon/logoff times for auditing and billing purposes.  This computer also provides web page services for http://confocal.med.unc.edu, and also provides a booking calendar.  The booking calendar is the means by which users can book time on the Leica Confocal system from anywhere with web access (on and off campus).

All computers are on the campus network.  Users' images may be accessed via the campus network using the following protocols: Appletalk, Novel Netware, MS-Windows (Netbeui), FTP. 

2.  Mission Statement

The facility provides a Confocal Laser Scanning Microscope system and know-how for high resolution confocal microscopic imaging, data processing and image analysis.  All users receive training on how to use the Leica Confocal tailored to their prior knowledge before being allowed to use to the system.  Training is directed  towards producing independent users.  Sample preparation is solely the users responsibility.  Advice is available but the facility personnel do not perform experiments, gather data for users or are responsible for scientific outcome.  Use of the confocal scanner is charged on a fee per unit time of use basis.  Currently users may come from anywhere in the UNC community.  Access to users from other institutions has been allowed when such users are collaborators with UNC personnel.

The facility is under the management of Dr. Michael Chua, with Dr. Robert Sealock as the senior faculty supervisor.  Dr. Neal Kramarcy prepares monthly usage reports and assists with user enquiries.  Hardware resources are nominally available from 7 am to 9 pm Monday to Friday and 7 am to 6pm Saturday and Sunday.  Users from outside of Cell & Molecular Physiology may borrow an access card to the Medical Sciences Research Building for short term after hours access.

Training in the independent use and proper care of the confocal microscope and the associated computer equipment and software, some advice in experimental design and assistance in optimal use of the equipment and image processing and handling are provided.  Once trained and checked out, users are allowed independent access to the equipment.  Training, which involves major contact time, is done on Mondays.  Simple user questions are answered as needed when time permits.

A World Wide Web booking calendar system is provided for the facility, and is available from any location to all authorized users (see http://confocal.med.unc.edu/scripts/display_bookings28.exe). World Wide Web pages concerning usage and information about the LeicaTCS-NT are available to all at http://confocal.med.unc.edu.

Dr. Chua & Dr. Kramarcy assure that the facility, including optical, mechanical, software, network and computer components, is an efficient, smoothly running facility.  Occasionally small programs are written by Dr. Chua, to assist in the handling of image data, especially multi-channel, z-series and time series data, and made available to authorized users of the facility.

The WindowsNT computers in the facility maintain extensive logs of usage of the confocal equipment and ancillary computers used for image processing.  The information from these logs is passed on to the Cell & Molecular Physiology Department business office, where personnel prepare bills for the confocal scanner time accrued by users.  PIs are billed via the usual UNC billing channels.

The Cell and Molecular Physiology Department houses the facility, provides a telephone and partial salary support to Dr. Michael Chua & Dr. Neal Kramarcy in return for carrying out services for the facility.  The Department also paid for the purchase of WindowsNT software and Visual Developer Software and a hard disk drive.  Accounting support and management of the trust fund is provided by the Cell & Molecular Physiology business office.

3.  Charges

Usage charges:

All users/trainees are charged at the same rate regardless of Laboratory/Department/Institution.

See http://confocal.med.unc.edu/wwwleica/Operation/Booking_Policy_Charges.html

4.  Location

The confocal microscope facility is housed by the Cell & Molecular Physiology Department in room 006 of the Medical Sciences Research Building.

For a map see http://confocal.med.unc.edu/wwwleica/Location/Location.html

5. Personnel

Dr. Michael Chua

mchuaREMOVETHIS@med.unc.edu

843-3268  966 6117 or 843 9469

Dr. Robert Sealock

sealockREOMVE@med.unc.edu

966 1272

Dr. Neal Kramarcy

kramarcy@med.unc.edu

966 1272

Notes

Initial capital funding for the Leica Confocal system was provided by a Research Resources for Medical Schools grant obtained in 1996 by Dean Michael Simmons, PI; Stanley C. Froehner, William Marzluff, and Oliver Smithies, co-applicants.

Further information please contact Dr. Michael Chua or Dr. Robert Sealock

Created 2000-02-23     updated 2007-07-19


Icon_Inverted_Laser.GIF (1500 bytes) Icon_book_white.gif (1143 bytes) 1998-2003 Michael Chua, Cell & Molecular Physiology, UNC.  All rights reserved. 
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