Basic Confocal Operation - Extended Focus


An extended focus image is created from a stack of serial z-sections.  The images are flatted into one 2-d layer.  This view differs from a thick section in that all the z-levels are in focus and a crisp image is formed of all the features throughout the volume.

 


Icon_Inverted_Laser.GIF (1500 bytes) Icon_book_white.gif (1143 bytes) 1998-2003 Michael Chua, Cell & Molecular Physiology, UNC.  All rights reserved. 
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