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1  Principles of Confocal Scanning

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1.5   The Noise Rubric

Obtaining a bright confocal image depends on several factors: 
dye concentration <-> laser power <-> pinhole size <-> PMT voltage <-> signal averaging
Each one of these factors can be optimized and involve practical limitations and compromises.  

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NEXT ---> 1.6   Dynamic Range & PMT Gain and Offset

Last updated 10/24/06


Icon_Inverted_Laser.GIF (1500 bytes) Icon_book_white.gif (1143 bytes) 1998-2003 Michael Chua, Cell & Molecular Physiology, UNC.  All rights reserved. 
To Confocal Homepage To Operating the confocal To Booking the confocal Go back a page Comments: Dr. Michael Chua